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Figure 1
Overall structures of the observed conformations of CaM. (a) Electron density at EF-hand 2 of the non-bent monomer of crystal form 2. The Sr atom is shown in green and the 2FoFc electron density is shown in cyan at a contour level of 1σ. The FoFc electron density at 4.0σ when Ca was refined at the position of the Sr atom is shown in magenta. (b) The two monomers in crystal form 2 coloured by temperature factor. (c) The canonical monomer, with a straight central helix, from crystal form 2 is shown in light blue, the bent monomer from crystal form 1 in shown in green and the bent monomer from crystal form 2 is shown in magenta. The structures were superimposed based on the residue range 68–78, i.e. between the N-terminal lobe and the site of bending. (d) A temperature-factor plot of the four CaM molecules in the current crystal forms. Straight and bent conformations of crystal form 1 are shown in black and green, respectively, and straight and bent conformations of crystal form 2 are shown in blue and magenta, respectively. (e) A comparison of the conformation of the straight helix (light blue), the bent monomer from crystal form 1 (green) and the original CaM structure with a small bend at Asp80 (yellow). It is clear that the bending is more pronounced in crystal form 1 than in the original CaM structure. For clarity, the view is rotated 180° about the y axis compared with that in (c).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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