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Figure 6
The interaction of CHES with the PLpro active site and its inhibitory effect. (a) Hydrogen-bonding (red dashed lines) and hydrophobic interactions between CHES (magenta) and PLpro (orange for palm domain and cyan for thumb domain). (b) The OMIT FoFc electron density for CHES at 1.4 Å resolution, contoured at 3.5σ. (c) Inhibition of PLpro by CHES. The experiments were performed in triplicate and the dots and bars represent the mean and errors, respectively. The curve is the best fit of the data for IC50 calculation. (d) Two inhibition patterns for CHES and 6TG. The points are the reciprocal of the experimental velocities, and the lines are the best fit of the data to (1)[link]. According to the results, the two inhibitors antagonized each other's binding to PLpro (α = 2.3).

Journal logoBIOLOGICAL
ISSN: 1399-0047
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