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Figure 6
(a) Multi-angle light-scattering detection of PpsRfull (straight line), PpsRΔHTH (dashed line), PpsRN-Q-PAS1 (dotted line) and PpsRQ-PAS1 (dashed/dotted line) fractionated by size-exclusion chromatography (individual traces are normalized according to the absorbance at 280 nm). The MALS-derived calculated molar-mass signals (grey) indicated on the right y axis yield average values of 150, 77, 52 and 14.3 kDa, respectively. (b) Absorbance traces of PpsRfull (black line) and PpsRΔHTH (grey dashed line) at 280 nm from the multi-angle light-scattering experiment in comparison to the absorbance trace of PpsRΔHTH (grey solid line) during preparative purification. The PpsRΔHTH concentration during preparative purification was about 30-fold higher compared with the multi-angle light-scattering experiment.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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