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Figure 7
Microscale thermophoresis measurements of the dimer–tetramer equilibria of PpsRfull (black line and squares) and PpsRΔHTH (light grey line and triangles) result in Kd values of 0.9 ± 0.3 and 0.9 ± 0.2 µM, respectively, based on the total dimer concentration. Measurements of the PpsRN-Q-PAS1 monomer–dimer equilibrium (dark grey line and filled circles) result in a Kd value of 0.25 ± 0.05 µM, based on the total monomer concentration. Error bars represent the standard deviation of three individual experiments.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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