ER of FD crystal structures. Ensemble structures are coloured by r.m.s.f., ranging from 0 to 5 Å; the r.m.s.f. was calculated per residue for all non-H atoms. (a) Wild-type FD (original PDB entry 1dsu
), with chains A (left) and B (right) plotted in blue and green, respectively; (b) R202A (R218A) FD mutant (original PDB entry 4cbn
), with chains A (left) and B (right) plotted in blue and green, respectively; (c) S183A (S195A) (original PDB entry 2xw9
) and the triple mutant of FD (original PDB entry 1dst
) shown on the left and right and plotted in blue and red, respectively. Coloured areas indicate the location of the 42–52 (59–65) flexible loop, the four exosite loops, spanning residues 132–135 (145–149) (L1), 155–159 (161–169) (L2), 173–176 (185–188) (L3) and 203–209 (219–224) (L4), and the self-inhibitory loop (SI). Models are coloured by r.m.s.f. from blue through green to red.