Figure 5
Thermal stability of BGLPf-WT (WT), BGLPf-M3 (M3), BGLPf-M4a (M4a), BGLPf-M4b (M4b) and BGLPf-M4c (M4c). The DSC results are shown as excess heat capacity, Cp (kcal K−1 mol−1), versus temperature (°C) profiles. The proteins (1.0 mg ml−1) were dissolved in 50 mM sodium phosphate buffer pH 7.0. The Tm temperatures of BGLPf-WT, BGLPf-M3, BGLPf-M4a, BGLPf-M4b and BGLPf-M4c were estimated as 109.5, 102.0, 74.0, 75.0 and 73.0°C, respectively. |