Role of κ→λ light-chain constant-domain switch in the structure and functionality of A17 reactibody

Ponomarenko, N.; Chatziefthimiou, S.D.; Kurkova, I.; Mokrushina, Yu.; Mokrushina, Y.; Stepanova, A.; Smirnov, I.; Avakyan, M.; Bobik, T.; Mamedov, A.; Mitkevich, V.; Belogurov, A.; Fedorova, O.S.; Dubina, M.; Golovin, A.; Lamzin, V.; Friboulet, A.; Makarov, A.A.; Wilmanns, M.; Gabibov, A.

doi:10.1107/S1399004713032446

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 1
Chemical compounds and antibody chains used in this study. (a) R = H, p-nitrophenyl 8-methyl-8-azabicyclo[3.2.1]octylphenylphosphonate (phosphonate X); R = biotin, biotinylated phosphonate X (BtX). (b) O,O-Diethyl O-(4-nitrophenyl)phosphate (paraoxon). (c) Amino-acid sequences of A17 κ and λ light chains and the heavy chain. Residues are numbered using the Kabat system (superlinear) and sequential numbering (interlinear). Frameworks (FR1–FR4) are underlined; switch residues of the J segment are designated as FR4 according to Kabat; CDRs are coloured magenta; constant domains κC_L, λC_L and C_H1 are coloured green, yellow and blue, respectively.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 70| Part 3| February 2014| Pages 708-719

doi:10.1107/S1399004713032446

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