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![[Figure 1]](be5254fig1mag.jpg)
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Figure 1
Biochemical characterization of the Uba1–Ub2 complex. (a) Native agarose gel electrophoresis documenting different migration patterns for Uba1 without Mg2+–ATP (lane 1), Uba1 with Mg2+–ATP (lane 2), Uba1–Ub without Mg2+–ATP (lane 3) and Uba1–Ub with Mg2+–ATP representing the Uba1–Ub2 complex (lane 4). (b) Analytical size-exclusion chromatography of Uba1–Ub (dashed line) and Uba1–Ub with Mg2+–ATP–ATP (solid line). (c) Crystals of the ternary Uba1–Ub2 complex with approximate dimensions of 60 × 70 × 10 µm. |
![[Figure 1]](be5254fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
