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![[Figure 5]](dw5088fig5_combined_mag.jpg)
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Figure 5
Mass-spectrometric results and the proposed mechanism of GTP hydrolysis. (a) Mass-spectrometric results of phosphate ion as a control and detected masses of GDP, GMP and sample phosphate from a sample of the GTP hydrolysis reaction in H218O. (b) Three different proposed mechanisms of GTP hydrolysis. The first line depicts the hydrolysis mechanism of GTP by attack on the α-phosphate by a H218O molecule. The second line depicts the hydrolysis mechanism of GTP and GDP by attack on the β-phosphate by a H218O molecule. The third line depicts the hydrolysis mechanism of GTP and GDP by attack on the γ-phosphate and the β-phosphate by a H218O> molecule, respectively. The products of each reaction are shown in the right column. The results in (a) indicate that the third mechanism is operational in hydrolysis by YmfB. |
![[Figure 5]](dw5088fig5_combined.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
