Figure 3
(a) σA-weighted Fobs − Fcalc electron-density map contoured at the 3σ level (grey) observed after soaking the apo LDH-A crystal form with NADH. The surrounding active-site region with residues lining the pocket shown as sticks is depicted in purple, with the modelled NADH depicted as yellow sticks. (b) Superposition of the crystal structures of the apo form (grey) and the NADH-bound binary complex (purple) of human LDH-A. The small-scale structural changes of the active-site loop and adjacent helix are indicated by an arrow. |