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Figure 1
Acoustic droplet ejection. Acoustic droplet ejection (ADE) uses sound energy to transfer variable micro-droplets (e.g. nanolitres or picolitres) of solution (protein, precipitant, fragments etc.) from a crystallization well, through a short air column (∼1 cm) to data-collection media. Sound-wave energy from the transducer is channeled to the focal point (i.e. ejection zone), displacing the surface where a controlled ejection occurs. Droplet size is governed by the wavelength of the sound emitted and this proportionality yields accurate ejected volumes. In this work, an Echo 550 liquid handler was used to co-crystallize proteins, precipitants and fragments in situ directly on MiTeGen MicroMeshes by vapour diffusion. The Echo 550 does not use frequency changes to transfer different volumes. Instead, it uses a fixed-frequency sound pulse to transfer each component in 2.5 nl increments.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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