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![[Figure 2]](nj5173fig2mag.jpg)
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Figure 2
Pin platform box. In situ vapor-diffusion co-crystallization is carried out in a pin platform box. All components are 3D printed (print files are available on request from the authors). The pin platform (A) contains 96 sockets for securing micromeshes. The lid (B) isolates the pin platform to prevent dehydration. The internal environment is governed by precipitant solution that is secured in 1% agar and is deposited into the moat (C). The window (D) is used to view specimens, to add components through apertures in the lid (E) and to remove excess mother liquor from the micromesh via apertures in the bottom of the pin platform box (Fig. 6 ![[link]](../../../../../../logos/arrows/d_arr.gif) ). The insets show a close-up view of the MiTeGen micromeshes above the window (including pink precipitant) and a magnified view of the in situ crystals. The distance between the agar in the moat and the meshes is 4 mm. The vapor-diffusion setup is equivalent to a sitting drop when the pin platform box is right side up; the box is inverted to achieve a hanging-drop configuration. |
![[Figure 2]](nj5173fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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