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Figure 3
In situ crystallization and fragment hit discovery directly on micromeshes. The pictures on the left show crystals grown in situ on the micromeshes. The pictures in the middle show electron density around known ligands (OMIT difference contoured at 3σ) that were co-crystallized with protein (N-­acetylglucosamine with lysozyme, L-aspartic acid with thermolysin, benzamidine with trypsin and L-proline with stachydrine demethylase). The pictures on the right show electron density around previously unreported fragment hits that were discovered using in situ co-crystallization (benzamidine with lysozyme, L-asparagine with thermolysin and imidazole with trypsin) and one unreported fragment (cleaved N-methylproline) that was previously discovered by one of the authors (see §[link]3.2). Note that the methyl moiety of N-methylproline is immediately cleaved in response to X-ray photoactivation of the enzyme (because of this, the methyl moiety is not shown in the figure). PDB codes are indicated for deposited structures.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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