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Figure 4
Placing multiple experiments on a single specimen holder allows the goniometer to act as an `auxiliary robotic automounter' that rapidly translates between experiments during the time that the conventional cryogenic automounter completes its duty cycle. (a) shows in situ co-crystallization of thermolysin, precipitant, cryoprotectant and fragments. In this example the fragments were asparagine (bottom), histidine (middle) and a control with no ligand (top). The custom specimen holder has sufficient space to accommodate ten discrete experiments (b) (with no ligands or cryoprotectants). Even with such a high density of specimens, the spacing between droplets is adequate to prevent cross-contamination (inset). A video is available on our website showing multiple crystals growing in situ directly on custom MiTeGen paddles, each with a different fragment (URL in §2.2[link]).

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ISSN: 2059-7983
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