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Figure 2
NCS rotamer consistency correction for IleA120 in PDB entry 1m0o at 1.7 Å resolution. (a) Starting orientation of IleA120 in 1m0o in the tt rotamer. Bad steric clashes (>0.4 Å) are depicted in hot pink. (b) χ angles adjusted to match the pt rotamer orientation of the NCS-related IleB120 side chain. (c) Result of the local conformation search, including backrub motion, shown in green. (d) Following a default run of phenix.refine, the correct pt rotamer is fitted to the density map. All maps are 2mFoDFc maps contoured at 1.2σ. Images were generated using KiNG (Chen et al., 2009BB8).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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