Figure 2
2Fo − Fc electron-density maps contoured at 1σ showing the AxCYTcp crystal structures of (a) ferric, (b) chemically reduced and (c) X-ray-reduced forms at 100 K. Additional X-ray-reduced structures determined at different temperatures are shown at 160 K (d) and 180 K (e). (f) Superposition of X-ray-reduced structures determined at 100 K (red), 160 K (grey) and 180 K (orange) with those of the chemically reduced (green) and ferric (blue) crystals. Note that in the chemically reduced, 160 and 180 K structures a minor occupancy conformation of Arg124 is present with density below the 1σ level (see Supplementary Fig. S3). |