 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](dw5092fig1mag.jpg)
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Figure 1
Adenosine nucleotide binding of hPrp28 determined by (a, b) fluorescence titration using mant-ADP or mant-ATP and (c) UV-induced cross-linking using α-32P-labelled ATP. (a, b) The difference in corrected fluorescence signal is plotted against the ADP/ATP concentration and fitted to the Michaelis–Menten equation. For mant-ADP, a Kd of 22.4 ± 2.4 µM was calculated, while in the case of mant-ATP curve fitting was not possible. (c) UV-induced cross-linking of α-32P ATP to purified hPrp28. 30 pmol purified hPrp28 was incubated in the absence of nuclear extract (Nxt; lanes 1–2) without (lane 1) or with (lane 2) poly-U (0.6 µg µl−1) or in the presence of nuclear extract (lanes 3–6) in the absence (lane 3) or presence (lane 4) of MINX pre-mRNA under splicing conditions (see § ![[link]](../../../../../../logos/arrows/d_arr.gif) 2) and subjected to UV irradiation. After cross-linking, hPrp28 was immunoprecipitated with anti-His antibody. The precipitates were loaded onto a 10% SDS–PAGE and radioactive hPrp28 (cross-linked to ATP) was detected by autoradiography. Cross-linking of ATP to hPrp28 was also assayed in the absence of UV irradiation (lane 5). As control for immunoprecipitation the reaction was carried out without purified hPrp28 (lane 6). |
![[Figure 1]](dw5092fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
