 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 7]](dw5099fig7mag.jpg)
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Figure 7
GARA for recombinant mC5a and mC5a-desArg against both hC5aR and mC5aR and comparison with commercial hC5a/hC5a-desArg. All results are indicated as the mean ± standard deviation of at least eight individual experiments (except for hC5a-desArg, where n = 4). The NAG release activity is expressed as a percentage of the maximal NAG release activity obtained with commercial hC5a. The statistical significance of the differential behaviour observed between hC5a and mC5a towards both receptors is as follows: p-value (hC5aR) = 0.0016; p-value (mC5aR) = 0.006. (a) Activation of hC5aR-expressing RBL cells by recombinant mC5a and mC5a-desArg compared with commercially available recombinant hC5a and hC5a-desArg. (b) As in (a) but with mC5aR-expressing RBL cells. (c) EC50 and maximal NAG release activity observed for all proteins upon incubation with hC5aR-expressing RBL cells. (d) As in (c) but with mC5aR-expressing RBL cells. |
![[Figure 7]](dw5099fig7.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
