 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
journal menu
![[Figure 4]](mh5125fig4mag.jpg)
|
|
Figure 4
Probing the dithiothreitol-dependent activity of (a) PfSHMT and (b) hSHMT. Proteins were prepared in the presence or absence of dithiothreitol and the assays were conducted in 50 mM HEPES pH 7.4 for PfSHMT or pH 7 for hSHMT, 0.5 mM EDTA omitting or including 1 mM dithiothreitol. For PfSHMT, 1 and 2 are activity traces of protein prepared, diluted and assayed in the system with dithiothreitol and without dithiothreitol, respectively, 3 and 4 are the activity traces of protein prepared in the absence of dithiothreitol diluted in buffer containing dithiothreitol for 30 and 5 min, respectively, and assayed in the system with dithiothreitol and 5 is theactivity trace of protein prepared and diluted in the system without dithiothreitol but assayed in the system with dithiothreitol. For hSHMT, (1) and (2) are activity traces of protein prepared, diluted and assayed in the system with dithiothreitol and without dithiothreitol, respectively. |
![[Figure 4]](mh5125fig4.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
