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![[Figure 1]](be5261fig1mag.jpg)
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Figure 1
Biological and enzymatic activity of recombinant CdAlr. (a) Coomassie Blue-stained reduced SDS–PAGE gel of recombinant CdAlrWT and CdAlrK271T following anion-exchange and size-exclusion chromatography. The arrow indicates the full-length molecular weight 43 303 bands corresponding to monomeric protein. The arrowheads indicate the cleavage products in CdAlrWT and lane M contains molecular-weight markers (labeled in kDa). (b) Growth rescue of a D-alanine auxotroph by plasmids producing recombinant CdAlrWT and CdAlrK271T. Growth or lack thereof of E. coli MB2159 (labeled a) and of E. coli MB2159 transformed with pUC57 vector (labeled b), wild-type C. difficile alr (labeled c) and C. difficile K271T mutant alr (labeled d) on LB medium supplemented with 50 µg ml−1 D-alanine (+ D-alanine) and LB medium without supplementation (− D-alanine). (c) Enzyme activity plots of purified recombinant CdAlrWT and CdAlrK271T. |
![[Figure 1]](be5261fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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