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Figure 3
Monomer–dimer distribution of NLRP14 PYD variants as probed by gel-filtration chromatography. Wild-type NLRP14 PYD (magenta) elutes at 12.04 and 13.46 ml, corresponding to dimeric (17%) and monomeric (83%) protein, respectively. NLRP14 PYD D86V (grey) elutes with a qualitatively similar profile (12.13 ml, 24%; 13.48 ml, 76%). By contrast, NLRP14 PYD L84R (cyan) exists exclusively as a monomer in solution (13.63 ml).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 70| Part 7| July 2014| Pages 2007-2018
https://doi.org/10.1107/S1399004714010311
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