 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](be5250fig3mag.jpg)
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Figure 3
Cartoon representation of the proposed conformational states accessed by AlgE as alginate is moved across the outer membrane by the double-gate model. The mechanism includes four major states: closed, open-in, open and open-out. These correspond to conformations where the P-loop (loop T8) and E-loop (loop L2) gates are in the open or closed positions with respect to the cationic selectivity pore demarked with plus signs. The exopolysaccharide alginate is indicated as a string of blue and white circles emanating from the alginate-synthesizing machinery at the inner membrane. AlgK is proposed to associate with alginate and with the P-gate of AlgE to direct the polymer into the open-in AlgE pore. The alginate-synthesizing and secretion complex of Alg8, Alg44, AlgG and AlgX is arranged based on a published model (Rehman et al., 2013  ). The peptidoglycan layer in the periplasmic space and AlgF, AlgI and AlgJ involved in alginate acetylation have been omitted for clarity. This cartoon is not drawn to scale. |
![[Figure 3]](be5250fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
