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Figure 3
ITC binding isotherm for the association of UNG with Ugi at 25°C in 20 mM sodium phosphate, 200 mM NaCl pH 7.5: the curves represent the nonlinear least-squares fit (lines) of the affinity (Kb), enthalpy change (ΔHb) and stoichiometry (n) obtained by fitting integrated heat pulse data (scattered points) to a single-site binding model. Key: hUNG, closed circles; cUNG, open circles.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 70| Part 8| July 2014| Pages 2093-2100
doi:10.1107/S1399004714011699
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