Figure 6
Elution profiles (UV absorption at 220 nm) of CPHPC from a reversed-phase column with acetonitrile gradients (acetonitrile, green line; aqueous, red line) at 4°C and pH values close to those employed in the crystallization of the protein complexes, showing three species corresponding to cis/cis (25%), cis/trans (45%) and trans/trans (30%) isomers at pH 4.5. |