Figure 3
Unit-cell volume dependence on aspects of cryomounting. (a) Dependence on the air-path length between the mounting microscope and the cryogen for three different protein crystals. Each data set is normalized to the unit-cell volume for the direct-to-oil plunge technique (0 m). Each plotted point is an average of 4–9 crystals with error bars ± standard error of the measured volumes. The transfer time is only approximate: more accurate transfer times are given in Supplementary Table S1. (b) Dependence on the value of the ambient humidity and crystal orientation in the loop for thaumatin crystals. Crystals were direct-mounted from the 1.2 m microscope position. Each data point is an average of 4–5 crystals, and the error bars show the standard error. `Parallel' and `perpendicular' refer to the orientation of the long axis of the crystal relative to the long axis of the loop. |