Figure 4
Dependence of the low-temperature thaumatin unit-cell volume on crystal size for four different cryomounting arrangements. All crystals were soaked in cryosolution (EG) for ∼2 min. Vial: crystals were equilibrated overnight in the vial and then mounted into the cryostream from the vial. Plunge: crystals were plunge-cooled in liquid nitrogen. Direct: crystals were directly mounted into the cryostream from two different microscope–cryostream distances. |