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Figure 7
The use of vapor equilibration to achieve cryoprotection of thaumatin crystals. All crystals were mounted on the diffractometer directly from the vial as described in §[link]2. (a, b) Crystals mounted directly from the growth drop and equilibrated in the vial over (a) the well solution for 45 min or (b) well solution (diluted to 80%) + 35%(w/v) methanol for 9 min. (c, d) Crystal soaked for 2 min in well solution + 10%(w/v) EG and equilibrated in a vial over (c) the same solution for 2 h or (d) well solution + 30%(w/v) EG for 45 min.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 70| Part 8| July 2014| Pages 2111-2124
doi:10.1107/S1399004714012310
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