Figure 7
Flash-induced absorption changes in aR2 at pH 6 (a, b) and pH 9 (c). (a) An aqueous suspension of claret membranes was excited with light pulses at 532 nm and absorption changes were recorded at various wavelengths. (b, c) The absorption kinetics in the time range from 0.003 to 90 ms were fitted with four components and the amplitude of each component was plotted against the wavelength of the light used for measurement. The dashed line represents the absorption change observed ∼7 µs after the excitation. |