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![[Figure 2]](tz5059fig2mag.jpg)
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Figure 2
Molecular replacement leads to an initial model of Pol I. (a) Surface representation of the Pol II model (PDB entry 1wcm) showing the five different regions used in the sequential molecular replacement, with non-included domains in grey. Structural domains are labelled. (b) Superposition of the initial Pol I model onto the Pol II structure taking Region 1 as a reference. The wide conformation of the Pol I cleft is clearly observed. (c) Anomalous difference Fourier map at 8 Å resolution, contoured at 4σ, showing the seven Zn positions in Pol I, two of which helped in the localization of A12.2. The Pol II model is shown as a grey ribbon. (d) Initial 12-subunit model of Pol I obtained by molecular replacement followed by homology modelling. Subunit colours are as shown in the inset. |
![[Figure 2]](tz5059fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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