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Figure 2
Integrated intensity versus dose for a 70S ribosome crystal at 300 K. A series of 20 80 ms exposures were first collected to probe for an ultrafast initial damage/intensity decay rate (solid circles, shown on an expanded dose scale at the bottom). A series of five 10 s exposures was then taken (solid squares) until the crystal was highly disordered (top). The frames could not all be integrated by a single software package. XDS/XSCALE was successful with the weakly exposed 80 ms frames, while only DENZO/SCALEPACK worked for the more fully exposed frames. Frames where both packages worked were used to put the two curves on the same scale. The initial diffraction resolution of the crystal was ∼5 Å. |