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![[Figure 7]](lv5073fig7mag.jpg)
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Figure 7
Stability of hRyR21–606 and its mutants. (a) Normalized thermal stability curves of hRyR21–606 (blue) and its mutants I419F (red), R420W (violet) and I419F/R420W (green), measured from 20 to 90°C. The transition around 38°C (indicated by the arrow) corresponds to the melting of Ile419 dimers. (b) Analytical SEC profile of hRyR21–606 wild type (blue) and of I419F (red), R420W (purple) and I419F/R420W (green) mutants. The elution maximum at 11.7 ml corresponds to dimers of I419F. Elution maxima at 12.9 ml correspond to the wild type and mutant monomers. (c)–(f) Far-UV CD spectra of hRyR21–606 wild type (c) and I419F (d), R420W (e) and I419F/R420W (f) mutants recorded at 4°C (black), 25°C (blue), 30°C (orange), 35°C (green) and 40°C (red). Insets show the ellipticities [Θ] (black) and the dynode voltage difference from the buffer baseline Δdyn (blue) at 218 nm as a function of the temperature. |
![[Figure 7]](lv5073fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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