Figure 4
Microscopic images of crystals anchored to mesh-type LithoLoops (Protein Wave Co.). All crystals are dyed with Izit dye (Hampton Research). (a) Top and side views of an anchored CesZ crystal. (b) The mounted crystal is automatically recognized. The green part (within 400 µm of the loop tip) is recognized as the crystal, the grey part is the loop and the red and blue prisms are the target points for the inkjet to eject the gel solution (red represents the active point currently ejected). (c) The same crystal as in (a) soaked in stabilization buffer. The thin layer around the crystal is gel. (d) Top and side views of an anchored GatCAB crystal. (e) Top and side views of an anchored lysozyme crystal. |