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Figure 7
Structural and biochemical implications for DNA substrate binding and catalysis. (a) Potential interactions of Lys146 in GBS_NucA (blue) with the 8-mer duplex DNA substrate from VVN nuclease (purple, PDB entry 1oup; Li et al., 2003BB24) compared with an arginine side chain (orange) at this position. (b) Specific GBS_NucA residues appear to be critical for DNA substrate binding. The 16-mer uncleaved duplex DNA substrate (scissile strand in orange, complementary strand in yellow) from VVN nuclease was modeled bound to GBS_NucA (blue ribbon). Side chains from GBS_NucA are shown in pink, with putative hydrogen-bonding interactions with the DNA substrate shown as black dashed lines.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 70| Part 11| November 2014| Pages 2937-2949
https://doi.org/10.1107/S1399004714019725
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