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![[Figure 7]](rr5081fig7mag.jpg)
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Figure 7
Ligation activity of Psy-Lig with different DNA substrates. (a) Schematic representation of DNA substrates and representative results of ligation on TBE–urea gels. The samples for the blunt substrate gel are for a 3.0 µM enzyme concentration over 8 h. The image contrast for the gapped substrate gel has been adjusted to allow visualization of the faint bands from ligation. Integration of bands was carried out prior to this adjustment. (b) Percentage of substrate ligated in 5 min for nicked, cohesive and mismatch substrates (main figure) and gapped substrate after 30 min (inset) as a function of enzyme concentration. (c) Percentage of blunt substrate ligated over 8 or 24 h for two Psy-Lig dilutions. Ligation activity was quantified by integration of band intensity and is expressed as the percentage of upper (ligated) band relative to the sum of the two bands. Measurements are the mean of three replicate experiments; error bars represent the standard deviation from the mean. |
![[Figure 7]](rr5081fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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