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![[Figure 8]](rr5081fig8mag.jpg)
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Figure 8
Kinetics of nick sealing by Psy-Lig and the effect of salt concentration measured using the MB assay. (a) The DNA (MB substrate) concentration was varied while the ATP concentration was kept at 0.1 µM. (b) The ATP concentration was varied while the DNA concentration was kept at 300 nM. (c) Activity as a function of MgCl2 concentration. The inset shows the low-concentration data range for clarity. (d) Activity as a function of NaCl concentration measured by the MB assay (solid line, circles) and the endpoint assay (dashed line, triangles). The measurements are the means of three replicate experiments; error bars represent the standard deviation from the mean. The values for the MB assay are the rates over the first 10 min of reaction, while the values for the endpoint assay are the ratio of ligated to unligated substrate. In (c) and (d) the data were normalized to the maximum rate under that condition and are expressed as a percentage. |
![[Figure 8]](rr5081fig8.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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