 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 5]](mn5072fig5mag.jpg)
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Figure 5
Effect of cations on the stability and activity of extralong MnP6 and its short variant. (a, b) Effect of 1 mM Ca2+ (green), Mn2+ (dark blue) or Cd2+ (cyan) and 0.5 mM Mn2+ + 0.5 mM Cd2+ (red) compared with a control without the above cations (magenta) on the stability of MnP6 after incubation in the pH range 2–8 for 24 h at 4°C (a) and in the 25–55°C range for 10 min at pH 5 (b). (c, d) Effect of cations on the pH (c) and thermal (d) stability of the MnP6 short variant assayed as described for (a) and (b). (e, f) Inverse plots of Mn2+ oxidation at pH 5.0 by the MnP6 short variant in the presence of 0 mM (black), 0.1 mM (red), 0.25 mM (green), 0.5 mM (blue) and 1.0 mM Cd2+ (magenta) (e) and ABTS oxidation at pH 4.5 in the presence of 0 mM (black), 5.5 mM (red), 15 mM (green) and 30 mM Cd2+ (blue) (f), revealing competitive inhibition in both cases. |
![[Figure 5]](mn5072fig5.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
