 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 7]](mn5075fig7mag.jpg)
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Figure 7
Acyl-CoA thioesterase activity of the wild-type (WT) and C92A and C92S,C382A mutant human mitochondrial 3-ketoacyl-CoA thiolase (hT1) enzymes measured by the production of CoA. (a) Acyl-CoA chain-length specificity of the wild-type (WT) and C92A variant hT1 measured with 100 µM of each substrate in the presence of 1 mM DTT. The data are shown as the mean ± s.d. of three experiments. (b) Effect of dithiothreitol (DTT) on the acyl-CoA thioesterase activity of WT and C92A and C92S,C382A variant hT1 with octanoyl-CoA. The activity was measured in the presence or absence of 1 mM DTT. The data are represented as the mean ± s.d. of two experiments. (c) Michaelis–Menten curve of the thioesterase activity of wild-type hT1 with octanoyl-CoA as substrate measured in the presence of 1 mM DTT. Data are shown as the mean ± s.d. of two to three experiments. The substrates used were C2-CoA, acetyl-CoA; C3-CoA, propionyl-CoA; C4-CoA, butyryl-CoA; C6-CoA, hexanoyl-CoA; C8-CoA, octanoyl-CoA; C10-CoA, decanoyl-CoA; C12-CoA, dodecanoyl-CoA; C14-CoA, tetradecanoyl-CoA. |
![[Figure 7]](mn5075fig7.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
