Figure 2
Flow diagram of NAMI, a program to analyse thermal shift assay data. Raw data are read in and analysed using a sliding window technique, where a window of increasing number of data points (5–35) is fitted by linear regression, resulting in a slope value (a) and a correlation coefficient (r) for each window. The optimal window size has been empirically found to be the one with the largest window size and r > 0.996. |