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Figure 2
Optimizing in meso crystallization of DgkA by varying the temperature, salt additives and host lipid. Details of how the optimizations were performed have been reported in Li, Shah et al. (2013BB41). (a) CLLD crystals grown at 20°C with 9.9 MAG as the host lipid and a precipitant consisting of 7.8%(v/v) MPD, 100 mM NaCl, 100 mM sodium citrate pH 5.6. (b) The same conditions as in (a) with crystallization at 4°C. (c) Nitrate was key to producing three-dimensional bipyramid-shaped crystals. The conditions are the same as in (b) with the inclusion of 100 mM LiNO3 in the precipitant solution. (d) CLLD DgkA crystals grown at 4°C with 7.8 MAG and a precipitant consisting of 5%(v/v) MPD, 100 mM NaCl, 100 mM LiNO3, 60 mM magnesium acetate, 50 mM sodium citrate pH 5.6.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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