 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](ba5218fig3mag.jpg)
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Figure 3
Characterization of SeMet-labelled CLLD DgkA. (a) Gel-filtration profile of SeMet CLLD. V0 and Vt indicate the void and total volume of the column, respectively. The elution volume for the protein is 74.9 ml. (b) Progress curve of the coupled assay reaction in the absence of protein (dotted line) and in the presence of CLLD (dashed line) and SeMet CLLD (solid line). Monoolein was used as the host lipid as well as the lipid substrate for the in meso assay of DgkA. (c) In meso crystals of SeMet CLLD grown at 4°C with 9.9 MAG and a precipitant solution consisting of 8.2%(v/v) MPD, 4%(v/v) 1,4-butanediol, 100 mM NaCl, 100 mM LiNO3, 100 mM sodium citrate pH 5.6. (d) In meso crystals of SeMet CLLD grown at 4°C with 7.8 MAG as the host lipid and a precipitant solution consisting of 4.5%(v/v) MPD, 100 mM NaCl, 60 mM magnesium acetate, 100 mM LiNO3, 50 mM sodium citrate pH 5.6. |
![[Figure 3]](ba5218fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
