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Figure 4
Representative results for heavy-atom co-crystallization trials. Trials were carried out using CLLD with 9.9 MAG (ae) and 7.8 MAG (fj) as host lipid at 4°C. The basic condition (without heavy atom) consisted of 7.8%(v/v) MPD, 100 mM NaCl, 100 mM LiNO3, 100 mM sodium citrate pH 5.6 for 9.9 MAG and of 5%(v/v) MPD, 100 mM NaCl, 100 mM LiNO3 60 mM magnesium acetate, 50 mM sodium citrate pH 5.6 for 7.8 MAG. The conditions with heavy atoms were as follows: (a) no heavy atom, (b) 0.1 mM mersalyl acid, (c) 0.1 mM K2PtCl4, (d) 0.5 mM NaAuCl4, (e) 0.5 mM Sm(NO3)3, (f) no heavy atom, (g) GdCl3, (h) 0.2 mM HgCl2, (i) 0.1 mM K2PtCl4 and (j) 0.5 mM Sm(NO3)3. Images (a)–(e) and (g)–(i) were recorded with normal light; images (f) and (j) were recorded between crossed polarizers.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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