Figure 8
In meso crystallization of single-Cys mutants of DgkA: (a) CM41, (b) CM42, (c) CM43, (d, e) CM62, (f) CM47, (g) CM102, (h) CM105. The crystallization conditions are 4–6%(v/v) MPD, 50–100 mM NaCl, 30–60 mM magnesium acetate, 100–200 mM LiNO3, 50 mM sodium citrate pH 5.6 for (a)–(d) and (f)–(h) and 5%(v/v) MPD, 100 mM LiNO3, 100 mM (NH4)2HPO4, 30 mM magnesium acetate, 50 mM sodium citrate pH 5.6 for (e). Crystals were grown at 4°C with 7.8 MAG as the host lipid. |