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Figure 4
Principle of light defocusing by a crystal. The focal volume of both objectives in air (n = 1.00) is represented by the black dot. When a crystal is present, each focal volume is translated to either the purple dot (white light objective) or the cyan dot (spectrophotometer objective) owing to refraction of light within the crystal (n > 1.00).

Journal logoBIOLOGICAL
ISSN: 1399-0047
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