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Figure 5
Optical artefacts in a coloured crystal. (a) Fluorescence excitation at the surface of a crystal. (b) Fluorescence excitation at the opposing face, with the emission light path traversing the whole thickness of the crystal. (c) Absorption (black) and emission spectra [red, geometry described in (a); blue, geometry described in (b); orange and green, intermediate situations] of a crystal of the cyan fluorescent protein Cerulean (Lelimousin et al., 2009BB55). (d) Absorption spectrum (black) of a crystal of Cerulean exhibiting a negative optical density around the maximum fluorescence emission (red).

Journal logoBIOLOGICAL
ISSN: 1399-0047
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