In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF

von Stetten, D.; Giraud, T.; Carpentier, P.; Sever, F.; Terrien, M.; Dobias, F.; Juers, D.H.; Flot, D.; Mueller-Dieckmann, C.; Leonard, G.A.; de Sanctis, D.; Royant, A.

doi:10.1107/S139900471401517X

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 7
Spectroscopic data corresponding to the proteins represented in Fig. 6

. (a, d) Comparison of reduced and oxidized proteins. (b, e) Photoreduction monitored by UV-vis absorption. (c) Buildup of a peroxo intermediate demonstrated by Raman spectroscopy using isotopically labelled hydrogen peroxide. The isotopic effect is indicated by purple arrows. (f) Comparison of the dark and meta-II intermediate states of bovine rhodopsin. (g) Comparison of absorption spectra in solution and crystalline states. (h) Photobleaching of a fluorescent protein monitored by fluorescence spectroscopy. (i) The presence of a disulfide bond probed by Raman spectroscopy. The position of the disulfide-bridge stretching mode is indicated by an arrow.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 71| Part 1| January 2015| Pages 15-26

doi:10.1107/S139900471401517X

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