Figure 4
Titration isotherm from data after automated thermogram processing using MicroCal Origin (blue circles) or NITPIC (red squares and error bars) and fit (red line) to a 1:1 binding model of a NITPIC isotherm (from Keller et al., 2012). The left inset shows the thermogram. The right insets illustrate the principle of baseline interpolation for three different injections as highlighted in red in the thermogram plot. |