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Figure 9
An example of detailed experimental planning for SV of a 2:1 binding system in SEDPHAT. (a) Parameter space of sw isotherms for the given binding system as functions of macromolecular concentration for species A (abscissa) and B (ordinate), both in logarithmic units, and with the color temperature indicating the theoretical sw values for given hypothesized binding constants, cropped to conditions where the total signal is between user-set parameters and experimentally feasible based on given stock concentrations. (b, c) Relative contribution of the AB and ABB complex, respectively, to the total signal. In all plots, a graphically user-generated trajectory (black line with points) is shown, which automatically generates the sample-preparation plan in (d).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 71| Part 1| January 2015| Pages 3-14
doi:10.1107/S1399004714010372
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