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Figure 1
Overview of current methods in biological cryo-EM. (a) Single-particle reconstruction of the TrpV channel (Liao et al., 2013BB29; EMD-5778). Left, projected views representing idealized single-particle data. Right, three-dimensional reconstruction of the mainly α-helical tetramer, coloured by subunit, with the fitted secondary structure. (b) Zernicke phase-contrast cryo-ET of a virus-infected cyanobacterial cell. Left, section through the tomogram; right, segmented view of the cell with the viruses in pink. Reproduced by permission from Macmillan Publishers Ltd , Dai et al. (2013BB9), copyright (2013). (c) Electron diffraction of lysozyme microcrystals. Left, optical micrograph of small crystals with microcrystals indicated by arrows. Right, representation of the three-dimensional electron diffraction data. Figures reproduced or modified from Shi et al. (2013BB46) under a Creative Commons Attribution license .

Journal logoBIOLOGICAL
ISSN: 1399-0047
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