 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 2]](ba5228fig2mag.jpg)
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Figure 2
An example of a CcpNmr Analysis project for the R3 talin rod domain. (a) Structural model of full-length talin showing all 18 talin domains. The structures of the domains shown in red were solved using the CcpNmr Analysis pipeline described in this report. (b) The fully assigned 1H–15N HSQC spectrum of R3. (c) The structure of the R3 talin rod domain. Left, ribbon drawing of a representative low-energy structure showing the overall topology of the four-helix bundle. The two vinculin binding helices are shown in blue. Right, superimposition of the 20 lowest energy structures consistent with the NMR data. (d–f) Screenshots of the three windows used to analyse and validate the results of the structure calculation. (d) The originating peak in the 13C HSQC-NOESY experiment; (e) the restraint shown on the structure colour-coded by the size of the violation; (f) the table of restraints, colour-coded by the fraction of structures violated. Colours range from green (satisfied) to red (violated). |
![[Figure 2]](ba5228fig2.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
