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Figure 8
Percentage variation of the background signal caused by deposition on the capillary surface. This plot takes the maximum observed variation in scattering intensity of the daily water-calibration measurements over a period of one month compared (as a percentage difference) with the scattering [s = 4πsin(θ)/λ] of a standard protein (calibration measurement of bovine serum albumin at a concentration of around 5 mg ml−1). Above s = 0.06 nm−1 the maximum variation attributed to contamination of the capillary is less than 0.3%. Below s = 0.06 nm−1 the effect of contamination rises to 0.6%, but as this signal will also be present in the background and thus accounted for in the subtraction, the actual effect on the data will be lower.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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